Description
| Product Name: | α-Glucosidase (α-GC) Activity Assay Kit |
| Product Code: | MAES0460 |
| Product Size: | 48T (32 samples) / 96T (80 samples) |
| Sample Type: | Tissue, fungal samples |
| Assay Time: | 30 min |
| Instrument: | Microplate reader (390-405 nm, optimal 400 nm) |
| Sensitivity: | 0.35 U/L |
| Detection Range: | 0.35-33.43 U/L |
| Recovery Rate: | 105% |
| Intra-assay CV: | 4.4-5.7% |
| Inter-assay CV: | 4.2-6.6% |
α-Glucosidase (α-GC), also known as α-D-glucoside hydrolase, is widely distributed in nature with extensive species diversity and varying properties, existing in virtually all organisms. It plays critical physiological roles in carbohydrate metabolism across animals, plants, and microorganisms. α-GC deficiency leads to severe glycogen metabolism disorders and excessive glycogen accumulation, causing Pompe disease (an autosomal recessive inherited glycogen storage disease, also known as glycogenosis type II). Chronic low α-GC activity in human tissues results in muscle fiber destruction and muscle atrophy.
This kit employs a detection principle whereby the chromogenic product generated from substrate hydrolysis catalyzed by α-glucosidase exhibits maximum absorption at 400 nm. The enzyme activity of α-GC is determined by measuring the optical density at 400 nm.
| Shelf Life: | 12 Months |
| Storage: | -20°C |
| Shipping: | Gel Pack |
| Research Area: | Enzymes, Carbohydrate Metabolism |
