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Glutathione Peroxidase Activity Assay Kit (MAES0084)

SKU:
MAES0084
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Cells, Cell Culture Supernatant, Tissue
Sensitivity:
17.17 U
Range:
17.17-518.32 U
Assay Time:
40 min
Shipping:
Gel Pack
Research Area:
Oxidative Stress
Antioxidant
Diabetes & Obesity
Plant & Environmental Stress
Enzyme Activity
$467
Frequently bought together:

Description

system_update_altDatasheet
Product Name:Glutathione Peroxidase Activity Assay Kit
Product Code:MAES0084
Product Size:96 Assays
Sample Type:Serum, plasma, cells, cell culture supernatant, tissue
Assay Time:40 min
Instrument:Microplate reader
Sensitivity:17.17 U
Detection Range:17.17-518.32 U
Recovery Rate:104
Inter CV:6.2
Inter CV:2.4

Glutathione peroxidase (GSH-Px) is an important enzyme that catalyzes decomposition of hydrogen peroxide. GSH specifically catalyze the reaction between GSH and hydrogen peroxide, protecting cell membrane structure and keeping membrane function integrity. Se-cysteine is the active center of the GSH-Px. Determination of GSH-Px activity in organism can be an indicator of selenium level as Se is essential section of GSH-Px.

Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH- Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.

Shelf Life:6 Months
Storage:2-8°C
Shipping:Gel Pack
Research Area:Enzymes, Oxidative stress

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