Glutathione Peroxidase Assay Kit
Glutathione peroxidase (GPX, EC 22.214.171.124) represents an enzyme family with peroxidase activity whose main biological role is to protect the organism from oxidative damage. It helps prevent lipid peroxidation of cellular membranes by removing free peroxide in the cell. GPX catalyzes the following reaction with glutathione reductase (GR), Simple, direct and high-throughput assays for GPX activity find wide applications.
Assay Genie' improved glutathione peroxidase assay directly measures NADPH consumption in the enzyme coupled reactions. The measured decrease in optical density at 340 nm is directly proportional to the enzyme activity in the sample.
For quantitative determination of glutathione peroxidase activity and evaluation of drug effects on GPX activity.
- Sensitive and accurate. Use 10 uL sample. Linear detection range 40 to 800 U/L GPX activity.
Glutathione Peroxidase Assay Kit Information:
|Kit Includes:||Assay Buffer: 25 mL GR Enzyme: 1 mL Glutathione: Dried NADPH: Dried Calibrator: Dried Peroxide Solution: 50 uL|
|Kit Requires:||Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, plate reader capable of reading optical density at 340 nm every minute, homogenizer etc.|
|Method of Detection:||OD340nm|
|Detection Limit:||40 U/L|
|Protocol Length:||20 min|
|Storage:||Store all components at -20°C.|
|Shelf Life:||6 months|