The GNB2 Monoclonal Antibody (CAB9643) is a high-quality antibody developed for reliable detection and analysis of target proteins. Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene contains a trinucleotide (CCG) repeat length polymorphism in its 5' UTR. RRID AB_2863746 Gene ID 2783 Swiss Prot Synonym SSS4; HG2C1; NEDHYDF; GNB2
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GNB2 Monoclonal Antibody
SKU:
CAB9643
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1675
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SSS4, HG2C1, NEDHYDF, GNB2
Positive Sample:
HeLa, NIH/3T3, Jurkat, SH-SY5Y, Rat brain
Cellular Localization:
Cytoplasm, Perinuclear Region, Cell Membrane.
Calculated MW:
37kDa
Observed MW:
35kDa
Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene contains a trinucleotide (CCG) repeat length polymorphism in its 5' UTR. RRID AB_2863746 Gene ID 2783 Swiss Prot Synonym SSS4; HG2C1; NEDHYDF; GNB2
Purification Method:
Affinity purification
Gene ID:
2783
RRID:
AB_2863746
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GNB2 Rabbit mAb (CAB9643) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using GNB2 Rabbit mAb (CAB9643) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
