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Anti-GNGT1 Antibody (CAB15675)

SKU:
CAB15675
Product Type:
Antibody
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 6 Anti-GNGT1 Antibody CAB15675
  • Cell Biology Antibodies 6 Anti-GNGT1 Antibody CAB15675
  • Cell Biology Antibodies 6 Anti-GNGT1 Antibody CAB15675
€139 - €419
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Description

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Antibody Name:Anti-GNGT1 Antibody
Antibody SKU:CAB15675
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-45 of human GNGT1 (NP_068774.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Mouse, Rat
Positive Samples:Mouse eye, Rat eye
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-45 of human GNGT1 (NP_068774.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPVI NIED LTEK DKLK MEVD QLKK EVTL ERML VSKC CEEV RDYV E
Gene ID:2792
Uniprot:P63211
Cellular Location:Cell membrane, Cytoplasmic side, Lipid-anchor
Calculated MW:8kDa
Observed MW:10kDa
Synonyms:GNGT1, GNG1
Background:This gene encodes the gamma subunit of transducin, a guanine nucleotide-binding protein (G protein) that is found in rod outer segments. Transducin, also known as GMPase, mediates the activation of a cyclic GTP-specific (guanosine monophosphate) phosphodiesterase by rhodopsin.
UniProt Protein Function:G-gamma t1: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. Belongs to the G protein gamma family.
UniProt Protein Details:

Protein type:G protein; G protein, heterotrimeric gamma; G protein, heterotrimeric

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: plasma membrane

Molecular Function:GTPase activity; protein binding

Biological Process: regulation of rhodopsin mediated signaling; rhodopsin mediated signaling

NCBI Summary:This gene encodes the gamma subunit of transducin, a guanine nucleotide-binding protein (G protein) that is found in rod outer segments. Transducin, also known as GMPase, mediates the activation of a cyclic GTP-specific (guanosine monophosphate) phosphodiesterase by rhodopsin. [provided by RefSeq, Jul 2016]
UniProt Code:P63211
NCBI GenInfo Identifier:52783592
NCBI Gene ID:2792
NCBI Accession:P63211.2
UniProt Secondary Accession:P63211,O43835, Q08447, Q16026, Q6LCP6, A4D1H2,
UniProt Related Accession:P63211
Molecular Weight:8,496 Da
NCBI Full Name:Guanine nucleotide-binding protein G(T) subunit gamma-T1
NCBI Synonym Full Names:G protein subunit gamma transducin 1
NCBI Official Symbol:GNGT1  
NCBI Official Synonym Symbols:GNG1  
NCBI Protein Information:guanine nucleotide-binding protein G(T) subunit gamma-T1
UniProt Protein Name:Guanine nucleotide-binding protein G(T) subunit gamma-T1
UniProt Synonym Protein Names:Transducin gamma chain
UniProt Gene Name:GNGT1  
UniProt Entry Name:GBG1_HUMAN
Anti-GNGT1 Antibody (CAB15675)Western blot analysis of extracts of various cell lines, using GNGT1 antibody (CAB15675) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-GNGT1 Antibody (CAB15675)Immunofluorescence analysis of rat eye using GNGT1 Polyclonal Antibody (CAB15675) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-GNGT1 Antibody (CAB15675)Immunofluorescence analysis of mouse eye using GNGT1 Polyclonal Antibody (CAB15675) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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