The Goat HSP 70 (Heat Shock Protein 70) ELISA Kit is specifically designed for the accurate and reliable detection of HSP 70 levels in goat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications. HSP 70 is a vital protein involved in cellular stress response, assisting in protein folding and preventing cellular damage under stressful conditions. It plays a key role in various biological processes, including protein quality control, immune response, and cell survival.
The measurement of HSP 70 levels can provide valuable insights into cellular stress and potentially aid in the understanding and treatment of conditions such as cancer, neurodegenerative diseases, and inflammatory disorders. With its advanced technology and superior performance, the Goat HSP 70 ELISA Kit is an essential tool for researchers looking to study the role of HSP 70 in cellular function and disease pathology. Get accurate and meaningful results with this innovative ELISA kit from Assay Genie.
Product Name:
Goat HSP-70 (Heat Shock Protein 70) ELISA Kit
SKU:
GTFI00031
Reactivity:
Goat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.469 ng/mL
Range:
0.781-50 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell Lysis Buffer or Tissue Lysis Buffer, and Other Biological Fluid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1
2
3
Serum (n = 5)
86-102%
85-104%
86-101%
EDTA Plasma (n = 5)
82-98%
86-96%
85-99%
Heparin Plasma (n = 5)
81-97%
81-92%
80-98%
Recovery:
Sample
1
2
Serum (n = 5)
88-102
96
EDTA Plasma (n = 5)
87-102
94
Heparin Plasma (n = 5)
85-103
93
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
