The Human HSP-70 (Heat Shock Protein 70) ELISA Kit is specifically designed for the precise measurement of HSP-70 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.HSP-70 is a key protein involved in cellular stress response, playing a vital role in protecting cells from damage and promoting cell survival. Dysregulation of HSP-70 has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human HSP-70 ELISA Kit provides researchers with a reliable tool to investigate the role of HSP-70 in disease pathogenesis and therapeutic development.
Product Name:
Human HSP-70 (Heat Shock Protein 70) ELISA Kit
SKU:
HUES02815
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
84-96
89-101
89-106
Average (%)
91
94
96
1:4
Range (%)
103-119
80-92
90-104
Average (%)
108
86
98
1:8
Range (%)
93-107
85-95
92-108
Average (%)
100
90
99
1:16
Range (%)
100-115
83-93
93-106
Average (%)
108
87
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
91-106
97
Cell Culture Media (n=8)
91-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.63
7.11
23.17
2.7
6.42
23.71
Standard deviation
0.14
0.29
1.35
0.17
0.39
2.09
C V (%)
5.48
4.06
5.84
6.36
6.01
8.83
Uniport ID:
P38646
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Human HSP-70/HSPA9 in samples. No significant cross-reactivity or interference between Human HSP-70/HSPA9 and analogues was observed
