The Mouse HSP 70 (Heat Shock Protein 70) ELISA Kit is specifically designed for precise and accurate detection of HSP 70 levels in mouse serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, guaranteeing dependable and consistent results for various research applications.HSP 70 is a key player in cellular stress response, helping to maintain protein homeostasis and protect cells from damage. Its role in various physiological and pathological processes, including cancer, inflammation, and neurodegenerative diseases, makes it a valuable biomarker for studying disease mechanisms and potential therapeutic interventions. With easy-to-follow protocols and user-friendly features, the Mouse HSP 70 ELISA Kit from Assay Genie is a reliable tool for researchers looking to investigate the role of HSP 70 in health and disease in mouse models.
Product Name:
Mouse HSP-70 (Heat Shock Protein 70) ELISA Kit
SKU:
MOES01127
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
23.44 pg/mL
Detection range:
39.06-2500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
86-97
86-96
96-108
Average (%)
91
91
102
1:4
Range (%)
94-107
85-98
95-111
Average (%)
101
92
102
1:8
Range (%)
97-110
82-97
92-104
Average (%)
103
88
97
1:16
Range (%)
100-112
86-100
92-102
Average (%)
106
93
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
97-111
102
Cell Culture Media (n=8)
88-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
117.99
284.42
1008.81
118.09
296.51
1040.27
Standard deviation
5.33
16.24
45.7
8.95
19.96
82.81
C V (%)
4.52
5.71
4.53
7.58
6.73
7.96
Uniport ID:
P38647
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Mouse HSP-70/HSPA9 in samples. No significant cross-reactivity or interference between Mouse HSP-70/HSPA9 and analogues was observed
