Anti-GOT1 Antibody (CAB5822)

Antibody Name:	Anti-GOT1 Antibody
Antibody SKU:	CAB5822
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 244-413 of human GOT1 (NP_002070.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, 293T, HepG2, A-549, HeLa, Mouse liver, Mouse kidney, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 244-413 of human GOT1 (NP_002070.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FVSE GFEF FCAQ SFSK NFGL YNER VGNL TVVG KEPE SILQ VLSQ MEKI VRIT WSNP PAQG ARIV ASTL SNPE LFEE WTGN VKTM ADRI LTMR SELR ARLE ALKT PGTW NHIT DQIG MFSF TGLN PKQV EYLV NEKH IYLL PSGR INVS GLTT KNLD YVAT SIHE AVTK IQ
Gene ID:	2805
Uniprot:	P17174
Cellular Location:	Cytoplasm
Calculated MW:	44kDa/46kDa
Observed MW:	46kDa

Synonyms:	GOT1, AST1, ASTQTL1, GIG18, cAspAT, cCAT
Background:	Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

UniProt Protein Function:	GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
UniProt Protein Details:	Protein type:Amino Acid Metabolism - arginine and proline; Transferase; EC 2.6.1.1; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - cysteine and methionine; EC 2.6.1.3; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - alanine, aspartate and glutamate Chromosomal Location of Human Ortholog: 10q24.1-q25.1 Cellular Component: lysosome; cytoplasm; nerve terminal; cytosol; nucleus Molecular Function:cysteine transaminase activity; carboxylic acid binding; phosphatidylserine decarboxylase activity; aspartate transaminase activity; pyridoxal phosphate binding Biological Process: glutamate metabolic process; oxaloacetate metabolic process; glutamate catabolic process to aspartate; response to glucocorticoid stimulus; aspartate biosynthetic process; glucose metabolic process; pathogenesis; 2-oxoglutarate metabolic process; gluconeogenesis; methionine salvage; cellular response to insulin stimulus; sulfur amino acid metabolic process; glycerol biosynthetic process; fatty acid homeostasis; carbohydrate metabolic process; aspartate catabolic process; aspartate metabolic process; amino acid biosynthetic process; glutamate catabolic process to 2-oxoglutarate; polyamine metabolic process Disease: Aspartate Aminotransferase, Serum Level Of, Quantitative Trait Locus 1
NCBI Summary:	Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]
UniProt Code:	P17174
NCBI GenInfo Identifier:	5902703
NCBI Gene ID:	2805
NCBI Accession:	P17174.3
UniProt Secondary Accession:	P17174,Q5VW80, B2R6R7, B7Z7E9,
UniProt Related Accession:	P17174
Molecular Weight:	Calculated MW: 44kDa/46kDaObserved MW: 46kDa
NCBI Full Name:	Aspartate aminotransferase, cytoplasmic
NCBI Synonym Full Names:	glutamic-oxaloacetic transaminase 1, soluble
NCBI Official Symbol:	GOT1
NCBI Official Synonym Symbols:	cCAT; GIG18; cAspAT; ASTQTL1
NCBI Protein Information:	aspartate aminotransferase, cytoplasmic; transaminase A; aspartate transaminase 1; aspartate aminotransferase 1; growth-inhibiting protein 18; cysteine transaminase, cytoplasmic; glutamate oxaloacetate transaminase 1; cysteine aminotransferase, cytoplasmic
UniProt Protein Name:	Aspartate aminotransferase, cytoplasmic
UniProt Synonym Protein Names:	Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A
Protein Family:	Protein transport protein
UniProt Gene Name:	GOT1
UniProt Entry Name:	AATC_HUMAN

	Western blot analysis of extracts of various cell lines, using GOT1 antibody (CAB5822) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat heart using GOT1 antibody (CAB5822) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using GOT1 antibody (CAB5822) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using GOT1 antibody (CAB5822) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using GOT1 antibody (CAB5822). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-GOT1 Antibody (CAB11363)	Human Aspartate aminotransferase, cytoplasmic (GOT1) ELISA Kit
GOT1 Antibody (PACO09539)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)