The GRHL1 Polyclonal Antibody (PAC061726) is a valuable tool for research involving the GRHL1 protein, a transcription factor that plays a crucial role in epithelial cell differentiation and maintenance. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting.GRHL1 is essential for maintaining the integrity of epithelial tissues, including the skin and the lining of organs such as the gut and lungs. Dysregulation of GRHL1 has been implicated in various diseases, including cancer and developmental disorders.
Therefore, studying the expression and function of GRHL1 can provide valuable insights into these conditions and potentially lead to the development of new therapeutic approaches.By utilizing the GRHL1 Polyclonal Antibody, researchers can effectively detect and analyze GRHL1 protein levels in different cell types, aiding in the investigation of its role in cell differentiation, tissue development, and disease progression. This antibody is a valuable tool for researchers in the fields of cell biology, developmental biology, and cancer research.
Western Blot. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate. All lanes: GRHL1 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 71, 50, 58 kDa. Observed band size: 71 kDa.
IHC image of PACO61726 diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO61726 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Transcription factor involved in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3'. Important regulator of DSG1 in the context of hair anchorage and epidermal differentiation, participates in the maintenance of the skin barrier. There is no genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity during epithelia development (By similarity). Isoform 1 may function as an activator and isoform 2 as a repressor in tissues where both forms are expressed.
GRHL1: Transcription factor. Isoform 1 may function as an activator and isoform 2 as a repressor in tissues where both forms are expressed. May play a role in development. May be involved in epidermal differentiation. Binds and transactivates the EN1 promoter. Belongs to the grh/CP2 family. Grainyhead subfamily. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Transcription factorChromosomal Location of Human Ortholog: 2p25.1Cellular Component: cytoplasm; Golgi apparatus; intracellular membrane-bound organelle; nucleoplasm; nucleusMolecular Function: chromatin DNA binding; sequence-specific DNA bindingBiological Process: epidermis development; positive regulation of transcription from RNA polymerase II promoter
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the grainyhead family of transcription factors. The encoded protein can exist as a homodimer or can form heterodimers with sister-of-mammalian grainyhead or brother-of-mammalian grainyhead. This protein functions as a transcription factor during development. [provided by RefSeq, Jun 2009]
