Anti-GRIA1 Antibody (CAB1826)

Antibody Name:	Anti-GRIA1 Antibody
Antibody SKU:	CAB1826
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-300 of human GRIA1 (NP_000818.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-300 of human GRIA1 (NP_000818.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ANFP NNIQ IGGL FPNQ QSQE HAAF RFAL SQLT EPPK LLPQ IDIV NISD SFEM TYRF CSQF SKGV YAIF GFYE RRTV NMLT SFCG ALHV CFIT PSFP VDTS NQFV LQLR PELQ DALI SIID HYKW QKFV YIYD ADRG LSVL QKVL DTAA EKNW QVTA VNIL TTTE EGYR MLFQ DLEK KKER LVVV DCES ERLN AILG QIIK LEKN GIGY HYIL ANLG FMDI DLNK FKES GANV TGFQ LVNY TDTI PAKI MQQW KNSD ARDH TRVD WKRP KYTS ALTY DGVK VM
Gene ID:	2890
Uniprot:	P42261
Cellular Location:	Cell junction, Cell membrane, Cell projection, Endoplasmic reticulum membrane, Multi-pass membrane protein, dendrite, dendritic spine, postsynaptic cell membrane, postsynaptic density, synapse
Calculated MW:	92kDa/93kDa/101kDa/102kDa
Observed MW:	110kDa

Synonyms:

GRIA1, GLUH1, GLUR1, GLURA, GluA1, HBGR1, GluR1

Background:

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	GluR1: an integral membrane protein belonging to the glutamate-gated ion channel family. L-glutamate (Glu) acts as an excitatory neurotransmitter at many synapses in the central nervous system. Glutamate receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. Each of the four GluR proteins (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing. Expressed in granule and pyramidal cells in the hippocampal formation.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass; Channel, calcium; Channel, ligand-gated Chromosomal Location of Human Ortholog: 5q31.1 Cellular Component: cell junction; cell soma; cell surface; dendrite; dendritic spine; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; Golgi membrane; plasma membrane; postsynaptic density; postsynaptic membrane; recycling endosome; synaptic vesicle Molecular Function:alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity; extracellular-glutamate-gated ion channel activity; glutamate receptor activity; PDZ domain binding; protein binding Biological Process: cellular protein metabolic process; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; ionotropic glutamate receptor signaling pathway; long-term memory; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine; receptor internalization; signal transduction; synaptic transmission
NCBI Summary:	Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P42261
NCBI GenInfo Identifier:	116242505
NCBI Gene ID:	2890
NCBI Accession:	P42261.2
UniProt Secondary Accession:	P42261,Q2NKM6, B7Z2S0, B7Z2W8, B7Z3F6, B7Z9G9, D3DQI4 E7ESV8,
UniProt Related Accession:	P42261
Molecular Weight:	102,864 Da
NCBI Full Name:	Glutamate receptor 1
NCBI Synonym Full Names:	glutamate ionotropic receptor AMPA type subunit 1
NCBI Official Symbol:	GRIA1
NCBI Official Synonym Symbols:	GLUH1; GLUR1; GLURA; GluA1; HBGR1
NCBI Protein Information:	glutamate receptor 1
UniProt Protein Name:	Glutamate receptor 1
UniProt Synonym Protein Names:	AMPA-selective glutamate receptor 1; GluR-A; GluR-K1; Glutamate receptor ionotropic, AMPA 1; GluA1
UniProt Gene Name:	GRIA1
UniProt Entry Name:	GRIA1_HUMAN

	Western blot analysis of extracts of BT-474 cells, using GRIA1 antibody (CAB1826) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat brain using GRIA1 antibody (CAB1826) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using GRIA1 antibody (CAB1826) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of rat brain using GRIA1 antibody (CAB1826) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse brain using GRIA1 antibody (CAB1826) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-Phospho-GRIA1-S849 Antibody (CABP0242)	GluR1 (Phospho-Ser863) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-GRIA1-S836 Antibody (CABP0356)	GluR1 Colorimetric Cell-Based ELISA Kit
Anti-Phospho-GRIA1-S845 pAb Antibody (CABP0825)
Anti-GluR1 Antibody (CAB11643)
Phospho-GRIA1 (S863) Antibody (PACO03964)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)