The [KO Validated] GSK3beta Antibody (CAB6164) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it an ideal choice for Western blot applications.GSK3B, also known as glycogen synthase kinase 3 beta, is a critical regulator of numerous signaling pathways and has been implicated in the development of various diseases including cancer, diabetes, and neurodegenerative disorders. By targeting GSK3B, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify novel therapeutic targets.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] GSK3beta Antibody
SKU:
CAB6164
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GSK3B, gsk-3β, GSK3β
Positive Sample:
LO2, HeLa, 22Rv1, U-251MG
Cellular Localization:
Cell Membrane, Cytoplasm, Nucleus.
Calculated MW:
47kDa
Observed MW:
46kDa
The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease.
Purification Method
Affinity purification
Gene ID
2932
RRID
AB_2863525
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using GSK3β Rabbit pAb (CAB6164) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunoprecipitation analysis of 200 μg extracts of 22Rv1 cells, using 3 μg GSK3β antibody (CAB6164). Western blot was performed from the immunoprecipitate using GSK3β antibody (CAB6164) at a dilution of 1:1000.
