Description
| Product Name: | GSSG Assay Kit - Colorimetric |
| Product Code: | MAES0086 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, animal tissue, red blood cells, cultured cells |
| Assay Time: | 100 min |
| Instrument: | Microplate reader |
| Sensitivity: | 0.36 µmol/L T-GSH |
| Detection Range: | 0.36-30 µmol/L T-GSH |
| Recovery Rate: | 97 |
| Inter CV: | 3.9 |
| Inter CV: | 0.6 |
Glutathione is a tripeptide (γ-L-glutamyl-L-Cysteinyl glycine) and the most common intracellular thiol. In cells, glutathione exists in two different forms, reduced (GSH) and oxidized (GSSG). Under physiological conditions, more than 98% of intracellular glutathione is GSH, because GSSG is rapidly reduced to GSH by glutathione reductase. GSH-GSSG system is the most abundant oxidation-reduction system in eukaryotic cells. It plays an important role in cell homeostasis and participates in apoptosis-related signal transduction.
GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle.
| Shelf Life: | 6 Months |
| Storage: | -20°C |
| Shipping: | Gel Pack |
| Research Area: | Oxidative stress |
