Hemoglobin Assay Kit (Colorimetric) (BA0043)

Product Type:
Detection Method:
Microplate Reader
Sample Type:
Blood, Serum, Plasma, Urine
Research Area:
Clinical Chemistry
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Hemoglobin Assay Kit - Information

Assay Genie's hemoglobin assay kit is based on an improved Triton/NaOH method, in which the hemoglobin is converted into a uniform colored end product. The intensity of color, measured at 400 nm, is directly proportional to hemoglobin concentration in the sample. The optimized formulation exhibits high sensitivity and is ideal for measuring hemolysis in low hemoglobin samples (e.g. serum and plasma).


For quantitative determination of hemoglobin and evaluation of drug effects on hemoglobin metabolism.

Hemoglobin Assay Kit - Key Features

  • Sensitive and accurate. Linear detection range 0.9 - 200 mg /dL hemoglobin in 96-well plate assay.
  • Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
  • Safety. Reagents are non-toxic.
  • Versatility. Assays can be executed in 96-well plate or cuvet.

Hemoglobin Assay Kit -Data Sheet

Kit IncludesHB Reagent: 50 mL Calibrator: 10 mL
Kit RequiresPipetting devices and accessories.
Method of DetectionOD400nm
Detection Limit0.9 mg/dL
SamplesBlood, serum, plasma, urine etc
Protocol Length5 min
Size250 tests
StorageStore reagent and calibrator at 4°C
Shelf Life12 months

More Details

HEMOGLOBIN (Hb) is made of four globin chains each carrying a heme group. It is carried by red blood cells and transports oxygen from the lungs to the peripheral tissues to maintain the viability of cells. Quantitation of blood hemoglobin has been a key diagnostic parameter for various diseases such as anemia, polycythemia and dehydration. Simple, direct and automation-ready procedures for measuring hemoglobin concentration are becoming popular in Research and Drug Discovery.