Hemoglobin Assay Kit (Colorimetric) (BA0043)
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Hemoglobin Assay Kit - InformationAssay Genie's hemoglobin assay kit is based on an improved Triton/NaOH method, in which the hemoglobin is converted into a uniform colored end product. The intensity of color, measured at 400 nm, is directly proportional to hemoglobin concentration in the sample. The optimized formulation exhibits high sensitivity and is ideal for measuring hemolysis in low hemoglobin samples (e.g. serum and plasma).
ApplicationsFor quantitative determination of hemoglobin and evaluation of drug effects on hemoglobin metabolism.
Hemoglobin Assay Kit - Key Features
- Sensitive and accurate. Linear detection range 0.9 - 200 mg /dL hemoglobin in 96-well plate assay.
- Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
- Safety. Reagents are non-toxic.
- Versatility. Assays can be executed in 96-well plate or cuvet.
Hemoglobin Assay Kit -Data Sheet
|Kit Includes||HB Reagent: 50 mL Calibrator: 10 mL|
|Kit Requires||Pipetting devices and accessories.|
|Method of Detection||OD400nm|
|Detection Limit||0.9 mg/dL|
|Samples||Blood, serum, plasma, urine etc|
|Protocol Length||5 min|
|Storage||Store reagent and calibrator at 4°C|
|Shelf Life||12 months|