Description
Whole Blood Hemoglobin Assay Kit (BA0062) (BA0062)
The Whole Blood Hemoglobin Assay Kit (SKU: BA0062) provides a simple, direct and automation-ready procedure for measuring total haemoglobin in whole blood. Haemoglobin is made of four globin chains, each carrying a heme group, and is carried by red blood cells to transport oxygen from the lungs to peripheral tissues. Quantitation of blood haemoglobin is a key diagnostic parameter for conditions such as anaemia, polycythaemia and dehydration. This kit is based on an improved aqueous alkaline method in which haemoglobin is converted into a uniform coloured end product, with the intensity measured at 570 nm being directly proportional to the haemoglobin concentration. The optimised formulation substantially reduces interference from substances in raw samples and uses no hazardous chemicals such as the potassium cyanide found in Drabkin's reagent.
| Product Name: | Whole Blood Hemoglobin Assay Kit (BA0062) |
| SKU: | BA0062 |
| Detection Method: | Colorimetric |
| Detection Range: | Determined against a 12.0 g/dL calibrator |
| Sample Type: | Whole blood |
| Species Reactivity: | All |
| Assay Time: | 5 minutes |
| Kit Size: | 250 Assays |
| Equipment Required: | Microplate reader |
| Storage: | 4C |
| Shelf Life: | 12 months after receipt |
| Shipping: | Room Temperature |
Quantitative colorimetric determination of haemoglobin in whole blood at 570 nm using an improved aqueous alkaline method that converts haemoglobin into a uniform coloured end product.
- Non-hazardous; no potassium cyanide or other hazardous chemicals used
- Simple mix-and-read procedure using a single reagent
- Can be performed in a 96-well plate or a cuvette
- High sensitivity using as little as 5 uL sample, ideal for limited samples such as mouse blood
- Direct assays of total haemoglobin in whole blood samples
- Pharmacology: effects of drugs on haemoglobin metabolism
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | 96-well plate procedure. Because 5 uL samples are used, perform assays in duplicate or triplicate where possible. Pipette 5 uL water (Blank), 5 uL Calibrator and 5 uL whole blood samples into wells of a clear-bottom 96-well plate. |
| 2 | Transfer 200 uL HB Reagent into all assay wells and tap to mix. |
| 3 | Incubate 5 minutes at room temperature and read OD at 570 nm (560-600 nm); OD values are stable for at least 60 minutes. |
| 4 | Cuvette procedure. Pipette 10 uL water (Blank), 10 uL Calibrator and 10 uL whole blood samples into separate cuvettes, transfer 1 mL HB Reagent into all cuvettes, mix, incubate 5 minutes at room temperature and read OD at 570 nm. |
Subtract the blank OD (water) from the Calibrator and Sample OD values. Haemoglobin = (ODsample - ODwater) / (ODcalibrator - ODwater) x [Calibrator], where the calibrator is 12.0 g/dL. Conversion: 1.0 g/dL Hb is equivalent to 0.156 mM.
| Component | Quantity | Storage |
| HB Reagent | 50 mL | 4C |
| Calibrator (12.0 g/dL) | 500 uL | 4C |
