The Glycogen synthase 1 (GYS1) Monoclonal Antibody (CAB8912) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Glycogen synthase 1 (GYS1) Monoclonal Antibody
SKU:
CAB8912
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1350
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GSY, GYS, Glycogen synthase 1 (GYS1)
Positive Sample:
CAL 27, Mouse skeletal muscle, Rat skeletal muscle
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
84kDa
Observed MW:
85kDa/84 kDa/84 kDa
The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
2997
RRID
AB_2863627
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from CAL 27 cells using Glycogen synthase 1 (GYS1) Rabbit mAb (CAB8912) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45 s.
Western blot analysis of various lysates using Glycogen synthase 1 (GYS1) Rabbit mAb (CAB8912) at 1:10000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): Mouse liver. Exposure time: 30 s.
