Anti-HAUS8 Antibody (CAB7847)

Antibody Name:	Anti-HAUS8 Antibody
Antibody SKU:	CAB7847
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 166-410 of human HAUS8 (NP_219485.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Jurkat, NCI-H460, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 166-410 of human HAUS8 (NP_219485.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NLAE FERR AEKN LLIM CKEK EKLQ KKAH ELKR RLLL SQRK RELA DVLD AQIE MLSP FEAV ATRF KEQY RTFA TALD TTRH ELPV RSIH LEGD GQQL LDAL QHEL VTTQ RLLG ELDV GDSE ENVQ VLDL LSEL KDVT AKKD LELR RSFA QVLE LSAE ASKE AALA NQEV WEET QGMA PPSR WYFN QDSA CRES GGAP KNTP LSED DNPG ASSA PAQA TFIS PSED FSSS SQAE VPPS LSRS GRDL S
Gene ID:	93323
Uniprot:	Q9BT25
Cellular Location:	Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, spindle, spindle pole
Calculated MW:	38kDa/44kDa
Observed MW:	41kDa

Synonyms:	HAUS8, DGT4, HICE1, NY-SAR-48
Background:

UniProt Protein Function:	HAUS8: Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Belongs to the HAUS8 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 19p13.11 Cellular Component: centrosome; cytosol Biological Process: centrosome organization and biogenesis; G2/M transition of mitotic cell cycle; spindle assembly
NCBI Summary:	HAUS8 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM, Jun 2010]
UniProt Code:	Q9BT25
NCBI GenInfo Identifier:	229462967
NCBI Gene ID:	93323
NCBI Accession:	Q9BT25.3
UniProt Secondary Accession:	Q9BT25,Q49AC4, Q86WF0, Q96FX3, B4DJA7, C9JBZ4,
UniProt Related Accession:	Q9BT25
Molecular Weight:	44,786 Da
NCBI Full Name:	HAUS augmin-like complex subunit 8
NCBI Synonym Full Names:	HAUS augmin like complex subunit 8
NCBI Official Symbol:	HAUS8
NCBI Official Synonym Symbols:	DGT4; HICE1; NY-SAR-48
NCBI Protein Information:	HAUS augmin-like complex subunit 8
UniProt Protein Name:	HAUS augmin-like complex subunit 8
UniProt Synonym Protein Names:	HEC1/NDC80-interacting centrosome-associated protein 1; Sarcoma antigen NY-SAR-48
Protein Family:	HAUS augmin-like complex
UniProt Gene Name:	HAUS8
UniProt Entry Name:	HAUS8_HUMAN

	Western blot analysis of extracts of various cell lines, using HAUS8 antibody (CAB7847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using HAUS8 antibody (CAB7847) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)