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Anti-Histone H2B Antibody (CAB1958)[KO Validated]

SKU:
CAB1958
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • KO Validated Antibodies 1 Anti-Histone H2B Antibody CAB1958KO Validated
  • KO Validated Antibodies 1 Anti-Histone H2B Antibody CAB1958KO Validated
  • KO Validated Antibodies 1 Anti-Histone H2B Antibody CAB1958KO Validated
  • KO Validated Antibodies 1 Anti-Histone H2B Antibody CAB1958KO Validated
  • KO Validated Antibodies 1 Anti-Histone H2B Antibody CAB1958KO Validated
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Description

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Antibody Name:Anti-Histone H2B Antibody [KO Validated]
Antibody SKU:CAB1958
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human HIST2H2BE (NP_003519.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SW480, BT-474, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human HIST2H2BE (NP_003519.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPEP AKSA PAPK KGSK KAVT KAQK KDGK KRKR SRKE SYSI YVYK VLKQ VHPD TGIS SKAM GIMN SFVN DIFE RIAG EASR LAHY NKRS TITS REIQ TAVR LLLP GELA KHAV SEGT KAVT KYTS SK
Gene ID:8349
Uniprot:Q16778
Cellular Location:Chromosome, Nucleus
Calculated MW:13kDa
Observed MW:15kDa
Synonyms:GL105, H2B, H2B.1, H2BFQ, H2BGL105, H2BQ, Histone H2B, HIST2H2BE
Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity.
UniProt Protein Function:H2B2E: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H2B family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: nucleoplasm; extracellular space; nucleosome; nucleus

Molecular Function:DNA binding; protein heterodimerization activity

Biological Process: nucleosome assembly; defense response to Gram-positive bacterium; establishment and/or maintenance of chromatin architecture; innate immune response in mucosa; antibacterial humoral response

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity. [provided by RefSeq, Aug 2015]
UniProt Code:Q16778
NCBI GenInfo Identifier:7387736
NCBI Gene ID:8349
NCBI Accession:Q16778.3
UniProt Related Accession:Q16778
Molecular Weight:14kDa
NCBI Full Name:Histone H2B type 2-E
NCBI Synonym Full Names:H2B clustered histone 21
NCBI Official Symbol:H2BC21  
NCBI Official Synonym Symbols:H2B; H2BQ; GL105; H2B.1; H2BFQ; H2BGL105; HIST2H2BE  
NCBI Protein Information:histone H2B type 2-E
UniProt Protein Name:Histone H2B type 2-E
UniProt Synonym Protein Names:Histone H2B-GL105; Histone H2B.q; H2B/q
Protein Family:Histone
UniProt Gene Name:HIST2H2BE  
UniProt Entry Name:H2B2E_HUMAN
Anti-Histone H2B Antibody [KO Validated] (CAB1958)Western blot analysis of extracts of various cell lines, using Histone H2B antibody (CAB1958) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-Histone H2B Antibody [KO Validated] (CAB1958)Western blot analysis of extracts from normal (control) and Histone H2B Rabbit pAb knockout (KO) 293T cells, using Histone H2B Rabbit pAb antibody (CAB1958) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 2s.
Anti-Histone H2B Antibody [KO Validated] (CAB1958)Immunohistochemistry of paraffin-embedded human esophagus using Histone H2B antibody (CAB1958) at dilution of 1:100 (40x lens).
Anti-Histone H2B Antibody [KO Validated] (CAB1958)Immunohistochemistry of paraffin-embedded rat kidney using Histone H2B antibody (CAB1958) at dilution of 1:100 (40x lens).
Anti-Histone H2B Antibody [KO Validated] (CAB1958)Immunohistochemistry of paraffin-embedded mouse liver using Histone H2B antibody (CAB1958) at dilution of 1:100 (40x lens).

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