The Histone H1.0 Monoclonal Antibody (CAB4342) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high specificity and sensitivity for human samples, making it ideal for use in various experimental techniques such as Western blotting.Histone H1.0 is a subtype of histone H1 that plays a crucial role in nucleosome organization and gene expression. Research suggests that histone H1.0 may be involved in the regulation of gene transcription and chromatin remodeling processes. By using the Histone H1.0 Rabbit Monoclonal Antibody (CAB4342), researchers can investigate the function and localization of histone H1.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Other (Wide Range Predicted) samples.
Product Name:
Histone H1.0 Monoclonal Antibody
SKU:
CAB4342
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Other (Wide Range Predicted)
Clone Number:
ARC1059
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
H10, H1.0, H1F0, H1FV, Histone H1.0
Positive Sample:
HeLa, HT-29, SH-SY5Y, Mouse testis
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
21kDa
Observed MW:
32kDa
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.
Purification Method
Affinity purification
Gene ID
3005
RRID
AB_2863238
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Histone H1.0 Rabbit mAb (CAB4342) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Histone H1.0 Rabbit mAb (CAB4342) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
