Description
HNF4alpha/gamma Transcription Factor Activity Assay
The HNF4alpha-Gamma Transcription Factor Activity Assay Kit is specifically designed for the sensitive and accurate detection of HNF4alpha and HNF4gamma transcription factor activity in cell lysates. This kit offers high sensitivity and specificity, providing reliable and reproducible results for researchers studying transcriptional regulation and gene expression.HNF4alpha and HNF4gamma are key transcription factors that play important roles in regulating gene expression involved in various biological processes, including metabolism, development, and cell differentiation. Dysregulation of HNF4alpha and HNF4gamma activity has been linked to various diseases, such as diabetes, liver disorders, and certain cancers, highlighting the importance of studying their activity.
With the HNF4alpha-Gamma Transcription Factor Activity Assay Kit, researchers can gain valuable insights into the modulation of gene expression by these transcription factors, leading to a better understanding of disease mechanisms and potential therapeutic targets. This kit is suitable for a wide range of applications in the field of molecular biology and can help advance research in diverse areas of biomedicine.
| Product Name: | HNF4alpha/gamma Transcription Factor Activity Assay |
| Product Code: | TFAB00182 |
| Target: | HNF4alpha/gamma |
| Synonyms: | Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, TCF-14, Transcription factor HNF-4 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie HNF4alpha/gamma transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | HNF4 alpha: a nuclear transcription factor which binds DNA as a homodimer. Controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic gene, alpha 1-antitrypsin, apolipoprotein CIII, and transthyretin genes. May be essential for development of the liver, kidney and intestine. Mutations have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Four alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Nuclear receptor; DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 20q13.12 Cellular Component: nucleoplasm; cytoplasm; nucleus Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; ligand-dependent nuclear receptor activity; protein binding; protein homodimerization activity; DNA binding; zinc ion binding; steroid hormone receptor activity; transcription factor activity; fatty acid binding; receptor binding Biological Process: transcription initiation from RNA polymerase II promoter; regulation of lipid metabolic process; intracellular receptor-mediated signaling pathway; positive regulation of transcription, DNA-dependent; lipid homeostasis; glucose homeostasis; endocrine pancreas development; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; xenobiotic metabolic process; regulation of gastrulation; response to glucose stimulus; ornithine metabolic process; gene expression; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; lipid metabolic process; negative regulation of cell growth; blood coagulation; regulation of insulin secretion; sex differentiation; phospholipid homeostasis Disease: Fanconi Renotubular Syndrome 4 With Maturity-onset Diabetes Of The Young; Maturity-onset Diabetes Of The Young, Type 1; Diabetes Mellitus, Noninsulin-dependent |
| NCBI Summary: | The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012] |
| UniProt Code: | P41235 |
| NCBI GenInfo Identifier: | 148886624 |
| NCBI Gene ID: | 3172 |
| NCBI Accession: | P41235.3 |
| UniProt Secondary Accession: | P41235,O00659, O00723, Q14540, Q5QPB8, Q6B4V5, Q6B4V6 Q6B4V7, Q92653, Q92654, A5JW41, B2RPP8, |
| UniProt Related Accession: | P41235 |
| Molecular Weight: | |
| NCBI Full Name: | Hepatocyte nuclear factor 4-alpha |
| NCBI Synonym Full Names: | hepatocyte nuclear factor 4, alpha |
| NCBI Official Symbol: | HNF4A |
| NCBI Official Synonym Symbols: | TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha |
| NCBI Protein Information: | hepatocyte nuclear factor 4-alpha; TCF-14; HNF4alpha10/11/12; transcription factor 14; transcription factor HNF-4; hepatic nuclear factor 4 alpha; nuclear receptor subfamily 2 group A member 1 |
| UniProt Protein Name: | Hepatocyte nuclear factor 4-alpha |
| UniProt Synonym Protein Names: | Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4 |
| Protein Family: | Hepatocyte nuclear factor |
| UniProt Gene Name: | HNF4A |
| UniProt Entry Name: | HNF4A_HUMAN |
