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Anti-HSD3B2 Antibody (CAB1823)

SKU:
CAB1823
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
  • Metabolism Antibodies 2 Anti-HSD3B2 Antibody CAB1823
  • Metabolism Antibodies 2 Anti-HSD3B2 Antibody CAB1823
€139 - €419
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Description

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Antibody Name:Anti-HSD3B2 Antibody
Antibody SKU:CAB1823
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of human HSD3B2 (NP_000189.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:Human, Mouse
Positive Samples:BT-474, U-937, COS1, COS-2, , Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of human HSD3B2 (NP_000189.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGWS CLVT GAGG LLGQ RIVR LLVE EKEL KEIR ALDK AFRP ELRE EFSK LQNR TKLT VLEG DILD EPFL KRAC QDVS VVIH TACI IDVF GVTH RESI MNVN VKGT QLLL EACV QASV PVFI YTSS IEVA GPNS YKEI IQNG HEEE PLEN TWPT PYPY SKKL AEKA VLAA NGWN LKNG DTLY TCAL RPTY IYGE GGPF LSAS INEA LNNN GILS SVGK FSTV NPVY VGNV AWAH ILAL RALR DPKK APSV RGQF YYIS DDTP HQSY DNLN YILS KEFG LRLD SRWS LPL
Gene ID:3284
Uniprot:P26439
Cellular Location:Endoplasmic reticulum membrane, Mitochondrion membrane, Single-pass membrane protein
Calculated MW:24kDa/42kDa
Observed MW:47kDa
Synonyms:HSD3B2, HSD3B, HSDB, SDR11E2
Background:The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:HSD3B2: protein localized to the nucleus in proliferating cells that seems to possess a tumor suppressor activity. Directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Suppresses carbonic anhydrase type II protein expression in carcinoid cell lines. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two differentially spliced isoforms have been identified.
UniProt Protein Details:

Protein type:Lipid Metabolism - androgen and estrogen; EC 1.1.1.145; Endoplasmic reticulum; Isomerase; Membrane protein, integral; Oxidoreductase; EC 5.3.3.1; Mitochondrial; Lipid Metabolism - C21-steroid hormone

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; mitochondrial membrane; mitochondrial inner membrane; integral to membrane; mitochondrial intermembrane space; smooth endoplasmic reticulum membrane

Molecular Function:steroid delta-isomerase activity; 3-beta-hydroxy-delta5-steroid dehydrogenase activity

Biological Process: steroid metabolic process; mineralocorticoid biosynthetic process; androgen biosynthetic process; glucocorticoid biosynthetic process; steroid biosynthetic process

Disease: 3-beta-hydroxysteroid Dehydrogenase, Type Ii, Deficiency Of

NCBI Summary:The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Code:P26439
NCBI GenInfo Identifier:112770
NCBI Gene ID:3284
NCBI Accession:P26439.2
UniProt Secondary Accession:P26439,Q16010, Q53GD4, Q6AI10, Q6LDB9, Q99890, Q9UD08 A2RRA5,
UniProt Related Accession:P26439
Molecular Weight:
NCBI Full Name:3 beta-hydroxysteroid dehydrogenase/Delta 5-->
NCBI Synonym Full Names:hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
NCBI Official Symbol:HSD3B2  
NCBI Official Synonym Symbols:HSDB; HSD3B; SDR11E2  
NCBI Protein Information:3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2; 3-beta-HSD II; 3 beta-HSD type II; progesterone reductase; delta 5-delta 4-isomerase type II; 3-beta-HSD adrenal and gonadal type; 3-beta-hydroxy-5-ene steroid dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; short chain dehydrogenase/reductase family 11E, member 2; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II; 3 beta-hydroxysteroid dehydrogenase type II, delta 5-delta 4-isomerase type II, 3 beta-HSD type II
UniProt Protein Name:3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
UniProt Synonym Protein Names:3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II; 3-beta-HSD II; 3-beta-HSD adrenal and gonadal typeIncluding the following 2 domains:3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)Alternative name(s):3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase
Protein Family:3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase
UniProt Gene Name:HSD3B2  
UniProt Entry Name:3BHS2_HUMAN
Anti-HSD3B2 Antibody (CAB1823)Western blot analysis of extracts of various cell lines, using HSD3B2 antibody (CAB1823) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-HSD3B2 Antibody (CAB1823)Immunofluorescence analysis of A-549 cells using HSD3B2 antibody (CAB1823).

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