SynonymsMCMP,MDC9,Mltng,Meltrin Gamma,Metalloprotease/disintegrin/cysteine-rich protein 9,Myeloma cell metalloproteinase,Cellular disintegrin-related protein
ReactivityHuman
Sample TypeTissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
SKU:
HUDL00065
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q13443
Sensitivity:
0.064ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MCMP,MDC9,Mltng,Meltrin Gamma,Metalloprotease/disintegrin/cysteine-rich protein 9,Myeloma cell metalloproteinase,Cellular disintegrin-related protein
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Human A Disintegrin And Metalloprotease 9 (ADAM9) ELISA Kit
The Human A Disintegrin and Metalloprotease 9 (ADAM9) ELISA Kit is specifically designed to accurately detect levels of ADAM9 in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.ADAM9 is a key protein involved in cell adhesion, migration, and proteolysis, making it a crucial player in processes such as tumor invasion, metastasis, and tissue remodeling. Elevated levels of ADAM9 have been linked to various diseases, including cancer, arthritis, and Alzheimer's disease, making it a valuable biomarker for studying and developing potential treatments for these conditions.
This ELISA kit provides researchers with a powerful tool for studying the role of ADAM9 in disease progression and for identifying potential therapeutic targets. Its ease of use, accuracy, and precision make it an indispensable resource for any laboratory conducting research in the field of molecular biology and biotechnology.
Product Name:
Human A Disintegrin And Metalloprotease 9 (ADAM9) ELISA Kit
Product Code:
HUDL00065
Size:
96 Assays
Target:
ADAM9
Synonyms:
MCMP, MDC9, Mltng, Meltrin Gamma, Metalloprotease/disintegrin/cysteine-rich protein 9, Myeloma cell metalloproteinase, Cellular disintegrin-related protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
