SynonymsMCMP,MDC9,Mltng,Meltrin Gamma,Metalloprotease/disintegrin/cysteine-rich protein 9,Myeloma cell metalloproteinase,Cellular disintegrin-related protein
ReactivityRat
Sample TypeTissue Homogenates, Cell Lysates And Other Biological Fluids
SKU:
RTDL00020
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q58GH6
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MCMP,MDC9,Mltng,Meltrin Gamma,Metalloprotease/disintegrin/cysteine-rich protein 9,Myeloma cell metalloproteinase,Cellular disintegrin-related protein
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Rat A Disintegrin And Metalloprotease 9 (ADAM9) ELISA Kit
The Rat ADAM9 (A Disintegrin and Metalloprotease 9) ELISA Kit is a powerful tool for the precise measurement of ADAM9 levels in rat samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and reproducible results, making it perfect for a variety of research applications.ADAM9 is a key enzyme involved in cell adhesion and migration, as well as the shedding of cell surface proteins.
Dysregulation of ADAM9 has been implicated in various diseases, including cancer, inflammatory disorders, and neurological conditions. As such, this ELISA kit is essential for studying the role of ADAM9 in these diseases and exploring potential therapeutic targets.Get your hands on the Rat ADAM9 ELISA Kit today and unlock new insights into the fascinating world of ADAM9 biology.
Product Name:
Rat A Disintegrin And Metalloprotease 9 (ADAM9) ELISA Kit
Product Code:
RTDL00020
Size:
96 Assays
Target:
ADAM9
Synonyms:
MCMP, MDC9, Mltng, Meltrin Gamma, Metalloprotease/disintegrin/cysteine-rich protein 9, Myeloma cell metalloproteinase, Cellular disintegrin-related protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
