The Human ACTG1 (Actin Gamma 1) CLIA Kit is specifically designed for the accurate measurement of ACTG1 levels in human samples such as serum, plasma, or cell culture supernatants. This kit is known for its high sensitivity and specificity, providing reliable and reproducible results that are essential for a variety of research applications.ACTG1, also known as gamma-actin, is a key protein that plays a crucial role in cell structure and motility. It is involved in processes such as cell division, migration, and contraction, making it a vital component in various cellular functions.
Dysregulation of ACTG1 has been linked to a wide range of diseases including cancer, cardiovascular disorders, and neurological conditions, highlighting its importance as a biomarker for the study and potential treatment of these diseases.With its superior performance and accuracy, the Human ACTG1 CLIA Kit is a valuable tool for researchers and clinicians looking to investigate the role of ACTG1 in disease pathology and explore potential therapeutic interventions. Order now and unlock the potential of ACTG1 in your research endeavors.
Product Name:
Human ACTG1 (Actin Gamma 1) CLIA Kit
SKU:
HUES00190
Target:
Human ACTG1 (Actin Gamma 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACTG1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACTG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACTG1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ACTG1. You can calculate the concentration of Human ACTG1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-110
101-119
93-107
Average (%)
100
108
98
1:4
Range (%)
90-102
89-99
94-108
Average (%)
96
94
101
1:8
Range (%)
90-104
94-109
100-113
Average (%)
95
100
106
1:16
Range (%)
99-113
92-108
97-113
Average (%)
105
100
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
96
EDTA plasma (n=5)
95-110
103
Cell culture media (n=5)
89-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.87
133.44
374.96
49.33
134.02
386.93
Standard deviation
4.94
15.68
42.03
5.34
11.24
31.65
C V (%)
9.71
11.75
11.21
10.83
8.39
8.18
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ACTG1 concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human ACTG1 in samples. No significant cross-reactivity or interference between Human ACTG1 and analogues was observed.
