The Human ADM (Adrenomedullin) CLIA Kit is a powerful tool for the quantitative measurement of adrenomedullin levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers precise and reliable results, making it an invaluable resource for a variety of research studies.Adrenomedullin is a vital peptide hormone that plays a crucial role in regulating various physiological processes, including vasodilation, angiogenesis, and inflammation. Dysregulation of adrenomedullin levels has been implicated in numerous pathologies, such as cardiovascular diseases, sepsis, and pulmonary hypertension, underscoring its significance as a diagnostic and therapeutic target.
By utilizing the Human ADM CLIA Kit, researchers can effectively investigate the role of adrenomedullin in health and disease, paving the way for the development of innovative treatment strategies and diagnostic tools. Elevate your research capabilities with this cutting-edge assay kit from Assay Genie.
Product Name:
Human ADM (Adrenomedullin) CLIA Kit
SKU:
HUES00204
Target:
Human ADM (Adrenomedullin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ADM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ADM. You can calculate the concentration of Human ADM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
98-111
101-116
Average (%)
96
105
107
1:4
Range (%)
97-109
92-105
87-98
Average (%)
104
98
93
1:8
Range (%)
96-112
87-102
100-117
Average (%)
102
94
108
1:16
Range (%)
89-100
93-106
91-107
Average (%)
95
99
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-115
108
EDTA plasma (n=5)
86-101
92
Cell culture media (n=5)
90-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.82
84.72
300.61
47.63
80.1
314.32
Standard deviation
4.6
7.48
21.01
5.59
7.42
31.68
C V (%)
10.5
8.83
6.99
11.74
9.26
10.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ADM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ADM in samples. No significant cross-reactivity or interference between Human ADM and analogues was observed.
