The Human ADM (Adrenomedullin) ELISA Kit is specifically designed for the precise measurement of adrenomedullin levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it a valuable tool for various research purposes.Adrenomedullin is a key regulatory peptide that plays a vital role in numerous physiological processes, including vasodilation, cardiovascular homeostasis, and anti-inflammatory effects. Dysregulation of adrenomedullin has been associated with various diseases such as sepsis, heart failure, and pulmonary hypertension, highlighting its significance as a biomarker for disease research and therapeutic development. The Human ADM ELISA Kit from Assay Genie provides researchers with a reliable and efficient method for measuring adrenomedullin levels in human samples, facilitating in-depth studies on the role of adrenomedullin in health and disease. With its reliable performance and user-friendly protocol, this kit is an indispensable tool for advancing scientific research in the field of adrenomedullin biology.
Product Name:
Human ADM (Adrenomedullin) ELISA Kit
SKU:
HUES01511
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
