The Human AlphaAFP (Alpha-Fetoprotein) ELISA Kit is specifically designed for the precise measurement of alpha-fetoprotein levels in human serum, plasma, and cell culture supernatants. This ELISA kit guarantees exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Alpha-fetoprotein is a key biomarker associated with various medical conditions, including liver cancer, fetal development abnormalities, and liver diseases. By detecting and quantifying alpha-fetoprotein levels, researchers can gain valuable insights into these conditions and potentially identify new treatment strategies.Overall, the Human AlphaAFP ELISA Kit is a valuable tool for studying alpha-fetoprotein and its implications in different disease states, ultimately contributing to advancements in medical research and clinical diagnosis.
Product Name:
Human alphaFP (Alpha-Fetoprotein) ELISA Kit
SKU:
HUES01357
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
