Human alphaFP (Alpha-Fetoprotein) Quickstep ELISA Kit
The QuickStep Human Alpha-Fetoprotein ELISA Kit is specifically designed for the precise measurement of alpha-fetoprotein levels in human samples such as serum and plasma. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Alpha-fetoprotein is a key biomarker associated with liver cancer, as well as other liver diseases and certain types of germ cell tumors. Monitoring alpha-fetoprotein levels can provide valuable insights into disease progression and treatment effectiveness, making this ELISA kit an invaluable tool for researchers and clinicians alike. With its user-friendly protocol and reliable performance, the QuickStep Human Alpha-Fetoprotein ELISA Kit is an essential addition to any laboratory conducting research on liver diseases and cancer. Order yours today to streamline your alpha-fetoprotein analysis and advance your research efforts.
Product Name:
Human alphaFP (Alpha-Fetoprotein) Quickstep ELISA Kit
SKU:
QSES029
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
1 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
