The Mouse AlphaFP (Alpha-fetoprotein) ELISA Kit is specifically designed for the precise measurement of alpha-fetoprotein levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research purposes.Alpha-fetoprotein is an important biomarker associated with liver development, liver disease, and certain types of cancer. Monitoring alpha-fetoprotein levels can provide valuable insights into these conditions, aiding in early detection and treatment strategies.Overall, the Mouse AlphaFP ELISA Kit is an essential tool for researchers studying liver health, cancer biology, and biomarker discovery in mouse models. With its reliable performance and versatile applications, this kit is a valuable asset for advancing scientific research in these areas.
Product Name:
Mouse alphaFP (Alpha-Fetoprotein) ELISA Kit
SKU:
MOES01686
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
