Description
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Human Angiogenesis Array (43 targets) (SARB0018)
The Human Angiogenesis Array (SARB0018) from Assay Genie is a comprehensive tool for studying the angiogenesis process in humans. This array allows for the simultaneous detection of 43 key proteins involved in angiogenesis, including growth factors, cytokines, and receptors. By utilizing this array, researchers can gain a better understanding of the complex molecular mechanisms underlying angiogenesis, a crucial process for tumor growth, wound healing, and cardiovascular development.The Human Angiogenesis Array provides a high level of sensitivity and specificity, making it suitable for a wide range of sample types, including serum, plasma, and cell culture supernatants.
The array is easy to use and offers reliable and reproducible results, making it an essential tool for studying angiogenesis in both basic research and clinical settings.Overall, the Human Angiogenesis Array from Assay Genie is a valuable resource for researchers looking to uncover the role of angiogenesis in various physiological and pathological conditions. With its comprehensive coverage of key angiogenic factors, this array has the potential to drive groundbreaking discoveries in the fields of cancer biology, vascular biology, and regenerative medicine.
| Product Sku: | SARB0018 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 43 |
| Gene Symbols: | ANG, ANGPT1, ANGPT2, CCL1, CCL13, CCL2, CCL5, CCL7, COL18A1, CSF2|CSF3, CXCL1, CXCL11, CXCL2, CXCL3, CXCL5, CXCL8, EGF, FGF2, FIGF, FLT4, IFNG, IGF1, IL10, IL1A, IL1B, IL2, IL4, IL6, KDR, LEP, MMP1, MMP9, PDGFB, PECAM1, PGF, PLAUR, PLG, TEK, TGFB1, THPO, TIMP1, TIMP2, TNF, TPO, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Angiogenin
| Angiopoietin-1
| Angiopoietin-2
| Angiostatin
| bFGF
|
EGF
| ENA-78 (CXCL5)
| Endostatin
| GCSF
| GM-CSF
|
GRO alpha/beta/gamma
| I-309 (TCA-3/CCL1)
| IFN-gamma
| IGF-1
| IL-10
|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
| IL-4
| IL-6
|
IL-8 (CXCL8)
| I-TAC (CXCL11)
| Leptin
| MCP-1 (CCL2)
| MCP-3 (MARC/CCL7)
|
MCP-4 (CCL13)
| MMP-1
| MMP-9
| PDGF-BB
| PECAM-1 (CD31)
|
PLGF
| RANTES (CCL5)
| TGF beta 1
| Thrombopoietin (TPO)
| Tie-2
|
TIMP-1
| TIMP-2
| TNF alpha
| uPAR
| VEGF-A
|
VEGFR2
| VEGFR3
| VEGF-D
| ||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Angiogenesis Antibody Array C1000 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
