The Human APOB (Apolipoprotein B) CLIA Kit is a cutting-edge diagnostic tool designed for the accurate measurement of apolipoprotein B levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an essential asset for a variety of research applications.Apolipoprotein B is a crucial protein involved in lipid transport and metabolism, playing a key role in conditions such as cardiovascular diseases and dyslipidemia. Detection of apolipoprotein B levels can provide valuable insights into an individual's risk for heart disease and guide personalized treatment strategies.This innovative CLIA kit offers researchers and healthcare professionals a powerful tool for studying the role of apolipoprotein B in health and disease, ultimately paving the way for improved diagnostics and therapies in the field of lipid disorders.
Product Name:
Human ApoB (Apolipoprotein B) CLIA Kit
SKU:
HUES00331
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
