The Mouse APOB (Apolipoprotein B) CLIA Kit is specifically designed for the precise measurement of apolipoprotein B levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Apolipoprotein B is a key protein involved in lipid metabolism and is essential for the transport of cholesterol and triglycerides in the bloodstream. Dysregulation of apolipoprotein B levels is associated with various metabolic disorders and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
This Mouse APOB CLIA Kit provides researchers with a reliable tool for investigating the role of apolipoprotein B in lipid metabolism and cardiovascular health in mouse models. Its easy-to-use format and robust performance make it an essential component for any laboratory studying lipid-related diseases.
Product Name:
Mouse ApoB (Apolipoprotein B) CLIA Kit
SKU:
MOES00084
Target:
Mouse ApoB (Apolipoprotein B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ApoB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ApoB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ApoB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ApoB. You can calculate the concentration of Mouse ApoB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-111
100-114
103-115
Average (%)
102
107
109
1:4
Range (%)
104-117
89-104
103-116
Average (%)
110
96
109
1:8
Range (%)
93-106
90-104
90-105
Average (%)
100
96
96
1:16
Range (%)
91-105
102-116
97-110
Average (%)
96
109
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
102
EDTA plasma (n=5)
101-113
107
Cell culture media (n=5)
85-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.25
48.76
170.48
22.18
48.93
183.5
Standard deviation
2.09
5.46
17.82
2.55
4.99
14.22
C V (%)
10.32
11.2
10.45
11.5
10.2
7.75
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ApoB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ApoB in samples. No significant cross-reactivity or interference between Mouse ApoB and analogues was observed.
