The Mouse ApoB (Apolipoprotein B) ELISA Kit is a cutting-edge assay designed for the precise and quantitative detection of Apolipoprotein B levels in a variety of mouse biological samples. Apolipoprotein B is a key structural protein in several lipoprotein particles, primarily low-density lipoprotein (LDL) known to be a critical player in lipid metabolism and atherosclerosis. As a crucial component of the lipoprotein particles that transport cholesterol in the bloodstream, ApoB has significant implications for cardiovascular health. Our Mouse ApoB ELISA Kit from Assay Genie is engineered to provide exceptional sensitivity and specificity, ensuring accurate and reproducible results for studies focused on lipid metabolism, cardiovascular diseases, and metabolic disorders. Manufactured under strict quality control standards, the kit offers robust performance and ease of use, making it an indispensable tool for researchers investigating the role of Apolipoprotein B in health and disease.
Product Name:
Mouse ApoB (Apolipoprotein B) ELISA Kit
SKU:
AEES00212
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
