The Human Arginase II (ARG2) ELISA Kit from AssayGenie is a reliable and efficient tool for the quantification of Arginase II levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an invaluable asset for a variety of research applications.Arginase II is an enzyme that plays a key role in the urea cycle, regulating the metabolism of arginine and impacting various physiological processes. Dysregulation of Arginase II has been implicated in conditions such as cancer, cardiovascular diseases, and inflammatory disorders, highlighting its significance as a potential biomarker for understanding these diseases and developing targeted therapeutic strategies.
With the Human Arginase II ELISA Kit, researchers can gain valuable insights into the role of Arginase II in disease pathology and therapeutic interventions, paving the way for advancements in personalized medicine and precision healthcare. Trust AssayGenie for accurate and reliable measurement of Arginase II levels in human samples, driving breakthrough discoveries in biomedical research.
Product Name:
Human Arginase II (Arg2) ELISA Kit
Product Code:
HUDL00237
Size:
96 Assays
Target:
Arg2
Synonyms:
Kidney-type arginase, Non-hepatic arginase
Detection Method:
Sandwich
Range:
3.125-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
85-103
94
Heparin plasma (n=5)
87-99
93
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
