The Human BAX (BCL-2 Associated X Protein) CLIA Kit is a highly sensitive assay designed for the quantitative measurement of BAX levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an invaluable tool for researchers studying apoptosis and cell death pathways.BAX is a key protein involved in the regulation of apoptosis, playing a crucial role in the activation of programmed cell death. Dysregulation of BAX has been implicated in various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions. By measuring BAX levels, researchers can gain valuable insights into the underlying mechanisms of these diseases and identify potential therapeutic targets.With its high sensitivity and specificity, the Human BAX CLIA Kit offers a reliable solution for quantifying BAX levels in human samples, allowing researchers to advance their understanding of apoptosis and its implications in disease development and progression.
Product Name:
Human Bax (Bcl-2 Associated X Protein) CLIA Kit
SKU:
HUES00384
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
