The Rat BAX (Bcl-2 Associated X Protein) ELISA Kit is a sophisticated assay crafted for the precise quantitative evaluation of BAX levels in various biological samples obtained from rats. BAX, a pivotal component of the BCL-2 protein family, is recognized for its pro-apoptotic role in modulating programmed cell death. This protein plays a crucial part in regulating the delicate balance between cell survival and apoptosis, thereby influencing cellular integrity and fate. Aberrations in BAX expression have been associated with diverse pathological conditions, emphasizing the significance of accurately measuring BAX levels in research settings. This ELISA kit offers outstanding sensitivity and specificity, ensuring the generation of dependable and reproducible data. With its stringent quality control measures, researchers can trust in the robust performance and user-friendly nature of the Rat BAX ELISA Kit. Whether investigating apoptotic pathways or exploring therapeutic targets, this kit serves as a reliable tool for unraveling the intricate mechanisms governed by BAX and its implications in various biological processes and disease states. Trust this kit to drive your research forward and unlock valuable insights into the role of BAX in cellular physiology and pathophysiology.
Product Name:
Rat BAX (Bcl-2 Associated X Protein) ELISA Kit
SKU:
AEES00323
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
