The Human BAX (BCL-2 associated X protein) ELISA Kit from Assay Genie is designed for the accurate quantification of BAX levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and consistent results, making it an invaluable tool for a variety of research applications. BAX is a key pro-apoptotic protein that plays a crucial role in regulating programmed cell death. Dysregulation of BAX has been implicated in various diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions. As such, BAX serves as a critical biomarker for understanding these diseases and developing potential therapeutic interventions. Invest in the Human BAX ELISA Kit from Assay Genie to advance your research and gain valuable insights into the role of BAX in health and disease.
Product Name:
Human Bax (Bcl-2 Associated X Protein) ELISA Kit
SKU:
HUES01746
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
