Description
Human BMP Related Array (8 targets) (SARB0026)
The Human BMP Related Array - 8 Targets (SARB0026) from Assay Genie is a powerful tool for researchers studying bone morphogenetic protein (BMP) signaling pathways. This array allows for the simultaneous detection of 8 different BMP-related targets in human samples, providing a comprehensive view of BMP pathway activity.This array includes antibodies against key BMP-related proteins such as BMP-2, BMP-4, BMP-6, BMP-7, SMAD1, SMAD5, Phospho-SMAD1, and Phospho-SMAD5. By measuring the levels of these proteins in a single experiment, researchers can quickly and easily analyze the activity of the BMP pathway in their samples.
The Human BMP Related Array - 8 Targets is designed for use in various applications, including Western blotting, immunoprecipitation, and ELISA. It is a valuable tool for studying the role of BMP signaling in development, tissue regeneration, and disease processes such as cancer and osteoporosis.With its high specificity and sensitivity, this array provides researchers with reliable and reproducible results, making it an essential resource for anyone working in the field of BMP signaling research.
| Product Sku: | SARB0026 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 8 |
| Gene Symbols: | BMP2, BMP4, BMP5, BMP6, BMP7, BMP8A, GDF11, GDF2 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
GDF-11 (BMP-11)
| BMP-2
| BMP-4
| BMP-5
| BMP-6
|
BMP-7
| BMP-8
| BMP-9
| ||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human BMP Related Array 2
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
