Human CD157/BST1 (MEN1112) Antibody is a research-grade biosimilar monoclonal antibody. It is a human IgG antibody. Reactivity: Human. Supplied as a liquid in 0.01M PBS pH 7.4. Applications: ELISA, flow cytometry, functional assays and in vivo research. Endotoxin: < 10 EU/mg. Purity: >95% purity as determined by SDS-PAGE. For research use only.
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2 (CD157/BST1) is a ~35 kDa protein. Catalyzes both the synthesis of cyclic ADP-beta-D-ribose (cADPR) from NAD(+), and its hydrolysis to ADP-D-ribose (ADPR). Cyclic ADPR is known to serve as an endogenous second messenger that elicits calcium release from intracellular stores, and thus regulates the mobilization of intracellular calcium (Probable). May be involved in pre-B-cell growth (Probable).
Product Name:
Human CD157/BST1 (MEN1112) Antibody
SKU:
GXBS00957
Size:
100 µg, 1 mg
Antibody Type:
Monoclonal Antibody
Clone:
MEN1112
Target:
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2
Isotype:
IgG
Host Species:
Human
Reactivity:
Human
Applications:
ELISA, FCM, Functional assay, Research in vivo
Expression Host:
Mammalian cells
Synonyms:
MEN1112
Biological Activity:
MEN1112 specifically binds to Bst1/CD157-positive cell lines with an EC 50 of 1 nM, and does not bind to Bst1/CD157-negative cell lines [1]. MEN1112 binds to recombinant Bst1/CD157 antigen with an EC 50 of 0.4 nM [1]. MEN1112 exhibits stronger high-affinity binding to both high- and low-affinity alleles of the CD16A Fc receptor on effector cells due to its defucosylated Fc domain [1]. MEN1112 induces slow internalization of the Bst1/CD157 antigen on target cells, a property that helps enhance its ADCC efficacy [1]. MEN1112 potently mediates antibody-dependent cell-mediated cytotoxicity (ADCC) against U937 acute myeloid leukemia (AML) cells, with an EC 50 of 30-140 pM [3]. MEN1112-mediated target cell lysis (20 h post-administration) is significantly enhanced upon the addition of 5% healthy donor serum to the co-culture system of healthy monocytes and autologous NK cells [3].
Purity:
>95% as determined by SDS-PAGE.
Endotoxin Level:
< 10 EU/mg
Formulation:
0.01M PBS pH 7.4.
State of Matter:
Liquid
Regulatory Status:
For research use only. Not suitable for clinical or therapeutic use.
Product Preparation:
Protein A/G purified from cell culture supernatant.
Storage and Handling:
Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
