The Human COL13 (Collagen Type 13) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of collagen type 13 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for research applications in various fields.Collagen type 13 is a crucial component of the extracellular matrix, playing a key role in maintaining tissue integrity and supporting cell adhesion.
Abnormal levels of collagen type 13 have been implicated in various diseases, including cancer, fibrosis, and connective tissue disorders, making it a valuable biomarker for studying these conditions and developing targeted therapies.With its high performance and ease of use, the Human COL13 CLIA Kit is an indispensable tool for researchers looking to investigate the role of collagen type 13 in health and disease.
Product Name:
Human COL13 (Collagen Type 13) CLIA Kit
SKU:
HUES00526
Target:
Human COL13 (Collagen Type 13)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL13. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL13, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL13. You can calculate the concentration of Human COL13 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
98-112
87-100
Average (%)
107
105
94
1:4
Range (%)
101-115
93-105
100-115
Average (%)
108
98
106
1:8
Range (%)
99-111
96-112
87-98
Average (%)
106
104
92
1:16
Range (%)
93-108
95-112
97-112
Average (%)
100
103
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-113
107
EDTA plasma (n=5)
101-118
108
Cell culture media (n=5)
96-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.37
267.18
870.95
100.52
263.29
870.02
Standard deviation
10.47
25.65
55.39
11.7
22.38
80.91
C V (%)
10.33
9.6
6.36
11.64
8.5
9.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL13 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL13 in samples. No significant cross-reactivity or interference between Human COL13 and analogues was observed.
