Description
Human Cytokine Array (23 targets) (SARB0034)
The Human Cytokine Array - 23 Targets (SARB0034) from AssayGenie is a powerful tool for researchers studying cytokine expression levels in human samples. This array allows for the simultaneous detection of 23 different cytokines, providing a comprehensive overview of the immune response in various cell types.With targets including interleukins, chemokines, and growth factors, this array enables researchers to analyze the complex interplay of cytokines in immune regulation, inflammation, and disease.
The array is easy to use, requiring only a small amount of sample for analysis, and produces reliable and reproducible results.Whether studying cancer, autoimmune disorders, or infectious diseases, the Human Cytokine Array - 23 Targets offers valuable insights into the cytokine profiles associated with different conditions. With its high sensitivity and specificity, this array is a valuable tool for any researcher seeking to unravel the intricate workings of the immune system.
| Product Sku: | SARB0034 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 23 |
| Gene Symbols: | CCL2, CCL5, CCL7, CCL8, CSF2, CSF3, CXCL1, CXCL8, CXCL9, IFNG|IL10, IL13, IL15, IL1A, IL2, IL3, IL5, IL6, IL7, LTA, TGFB1, TNF |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
GCSF
| GM-CSF
| GRO alpha/beta/gamma
| GRO alpha (CXCL1)
| IL-1 alpha (IL-1 F1)
|
IL-2
| IL-3
| IL-5
| IL-6
| IL-7
|
IL-8 (CXCL8)
| IL-10
| IL-13
| IL-15
| IFN-gamma
|
MCP-1 (CCL2)
| MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
| MIG (CXCL9)
| RANTES (CCL5)
|
TGF beta 1
| TNF alpha
| TNF beta (TNFSF1B)
| ||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
