Description
system_update_altDatasheet
Human Cytokine Array (32 targets) (SARB0035)
The Human Cytokine Array (SARB0035) is a powerful tool for researchers studying cytokine profiles in human samples. This array features 32 different cytokine targets, allowing for comprehensive analysis of inflammatory and immune responses in various biological systems.With its high sensitivity and specificity, this cytokine array provides accurate and reliable data for biomarker discovery, disease diagnosis, and drug development. Researchers can conveniently analyze multiple cytokines simultaneously, saving time and resources compared to traditional ELISA methods.
Whether investigating immune responses in cancer, autoimmune diseases, or infectious conditions, the Human Cytokine Array (SARB0035) offers valuable insights into the complex interplay of cytokines in various disease states. This array is a valuable tool for advancing research in immunology, oncology, and inflammatory disorders.
| Product Sku: | SARB0035 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 32 |
| Gene Symbols: | AHSG, ALB, AMICA1, ANGPT4, CD163, CLU, CRTAM, CSF3R, CST3, CXCL14|DLL1, FGF1, FOLR1, GPRASP1, GPRASP2, IL17RB, IL27, LAG3, LDLR, PGA3, PGA4, PGA5, PRAP1, RBP4, SDC1, SOST, SPINT2, TFPI, THBS1, TNFRSF13B, TNFSF11, TNFSF13B, TNNI2, WISP1 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
aFGF (FGF-1)
| Albumin
| AMICA
| Angiopoietin-4
| BAFF
|
CD163
| Clusterin
| CRTAM
| CXCL14 (BRAK)
| Cystatin C
|
DLL1
| Fetuin A
| FOLR1
| GASP-1 (WFIKKNRP)
| GASP-2 (WFIKKN)
|
GCSF R (CD114)
| HAI-2
| IL-17 RB
| IL-27
| LAG-3
|
LDL R
| Pepsinogen 1
| RBP4
| SOST
| Syndecan-1
|
TACI (TNFRSF13B)
| TFPI
| Thrombospondin 1
| TRANCE (TNFSF11)
| Troponin I
|
uPA
| WISP-1 (CCN4)
| |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C12 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
