Description
system_update_altDatasheet
Human Cytokine Array (49 targets) (SARB0032)
The Human Cytokine Array (49 targets) (SARB0032) from AssayGenie is a powerful tool for researchers studying the immune system and cytokine signaling pathways. This array kit allows for the simultaneous detection of 49 different cytokines, growth factors, and chemokines in human samples, providing comprehensive insight into the inflammatory and immune response. With its high sensitivity and specificity, this array kit enables researchers to accurately measure the levels of various cytokines in complex biological samples, allowing for the identification of potential biomarkers and therapeutic targets.
The kit is easy to use and provides reliable results, making it suitable for a wide range of applications in immunology, cancer research, and drug discovery.Overall, the Human Cytokine Array (49 targets) (SARB0032) is a valuable tool for researchers looking to further explore the role of cytokines in health and disease, and advance our understanding of immune system dynamics.
| Product Sku: | SARB0032 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 49 |
| Gene Symbols: | ACE2, AFP, ANGPT1, ANGPTL4, B2M, CA (19-9), CA9, CALCA, CCL14, CCL21|CD40LG, CDH1, CEACAM1, CEACAM5, CGA, CGB, CRP, CTSS, DCN, DKK1, DKK3, DKK4, DPP4, EDA, EPCAM, EPOR, ERBB2, FCER2, FSHB, HBEGF, IL13RA1, IL17B, IL17C, IL17F, IL17RA, KLK3, MUC1, MUC16, PARN, PLG, PROK1, RPS27A, S100B, SHH, TDGF1, TG, TGFBI, TNFRSF14, TNFRSF17, TNFRSF9, TNFSF9 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
4-1BB (TNFRSF9/CD137)
| ACE-2
| Alpha-fetoprotein
| Angiopoietin-1
| Angiostatin
|
ANGPTL4
| Beta-2 Microglobulin
| BCMA (TNFRSF17)
| beta IG-H3
| CA125
|
CA15-3
| CA19-9
| CA9
| Cathepsin S
| HCC-1 (CCL14)
|
6Ckine (CCL21)
| CD23
| CD40 Ligand (TNFSF5)
| CEA
| CEACAM-1
|
Cripto-1
| CRP (C-Reactive Protein)
| DAN
| Decorin
| DKK-1
|
Dkk-3
| Dkk-4
| CD26 (DPPIV)
| E-Cadherin
| EDA-A2
|
EG-VEGF (PK1)
| TROP1 (EpCAM)
| ErbB2
| Erythropoietin R
| FSH
|
HB-EGF
| hCG intact
| HVEM (TNFRSF14)
| IL-13 R1 | IL-17B
|
IL-17C
| IL-17F
| IL-17 RA
| Procalcitonin
| PSA-free
|
S100 B
| Sonic Hedgehog N-Terminal (Shh-N)
| Thyroglobulin
| Ubiquitin+1
| |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C10 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
