Description
system_update_altDatasheet
Human Cytokine Array (60 targets) (SARB0041)
The Human Cytokine Array (SARB0041) from Assay Genie is a powerful tool for researchers studying cytokine signaling pathways and immune responses. This comprehensive array allows for the simultaneous detection of up to 60 different cytokines, providing a broad view of the inflammatory and immune response profile in a sample.With high sensitivity and specificity, this array enables researchers to analyze cytokine levels in various sample types, including serum, plasma, and cell culture supernatants. The array is user-friendly, with easy-to-follow protocols and rapid results, making it suitable for high-throughput screening in both basic research and clinical studies.
Researchers can gain valuable insight into the complex interactions between cytokines and immune cells, aiding in the discovery of potential biomarkers for diseases such as cancer, autoimmune disorders, and infectious diseases. By uncovering the cytokine profiles associated with different conditions, researchers can identify new therapeutic targets and strategies for intervention.Overall, the Human Cytokine Array from Assay Genie is a versatile tool that empowers researchers to explore the intricate network of cytokine signaling in the immune system, advancing our understanding of immune regulation and disease pathogenesis.
| Product Sku: | SARB0041 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 60 |
| Gene Symbols: | ANG, BDNF, BMP4, BMP6, CCL1, CCL11, CCL13, CCL15, CCL17, CCL18|CCL2, CCL20, CCL22, CCL23, CCL24, CCL26, CCL5, CCL7, CCL8, CNTF, CSF1, CSF2, CX3CL1, CXCL12, CXCL13, CXCL6, CXCL9, EGF, FGF6, FGF7, FLT3LG, GDNF, IFNG, IGF1, IGFBP1, IGFBP2, IGFBP4, IL10, IL13, IL15, IL16, IL1A, IL1B, IL1RN, IL2, IL3, IL4, IL5, IL6, IL7, KITLG, LEP, LTA, NTF3, PDGFB, PPBP, TGFB1, TGFB3, TNF, TNFSF14 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Angiogenin
| BDNF
| BLC (CXCL13)
| BMP-4
| BMP-6
|
Ck beta 8-1 (CCL23)
| CNTF
| EGF
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
|
Eotaxin-3 (CCL26)
| FGF-6
| FGF-7 (KGF)
| Flt-3 Ligand
| Fractalkine (CX3CL1)
|
GCP-2 (CXCL6)
| GDNF
| GM-CSF
| I-309 (TCA-3/CCL1)
| IFN-gamma
|
IGFBP-1
| IGFBP-2
| IGFBP-4
| IGF-1
| IL-10
|
IL-13
| IL-15
| IL-16
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
|
IL-1 ra (IL-1 F3)
| IL-2
| IL-3
| IL-4
| IL-5
|
IL-6
| IL-7
| Leptin
| Light (TNFSF14)
| MCP-1 (CCL2)
|
MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
| MCP-4 (CCL13)
| M-CSF
| MDC (CCL22)
|
MIG (CXCL9)
| MIP-1 delta (CCL15)
| MIP-3 alpha (CCL20)
| NAP-2 (PPBP/CXCL7)
| NT-3
|
PARC (CCL18)
| PDGF-BB
| RANTES (CCL5)
| SCF
| SDF-1 alpha (CXCL12 alpha)
|
TARC (CCL17)
| TGF beta 1
| TGF beta 3
| TNF alpha
| TNF beta (TNFSF1B)
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C6 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
