Description
system_update_altDatasheet
Human Cytokine Array (80 targets) (SARB0040)
The Human Cytokine Array - 80 Targets (SARB0040) is a comprehensive tool for researchers studying the intricate network of cytokines involved in immune responses and inflammatory processes. This array kit includes 80 unique cytokine targets, allowing for the simultaneous detection and quantification of key signaling molecules involved in immune regulation.With a focus on high sensitivity and specificity, this array provides reliable and reproducible data for analyzing cytokine expression profiles in various biological samples. Whether you are investigating immune cell function, studying disease mechanisms, or evaluating therapeutic responses, this array offers a powerful solution for profiling cytokine patterns in a high-throughput manner.Designed for use in a variety of experimental settings, this multiplex array is compatible with different sample types, including cell culture supernatants, serum, plasma, and other biological fluids.
By measuring multiple cytokines in a single assay, researchers can gain valuable insights into the complex interplay of signaling molecules that drive immune responses and inflammatory processes.Overall, the Human Cytokine Array - 80 Targets (SARB0040) is a versatile and reliable tool for researchers seeking a comprehensive analysis of cytokine expression patterns. With its high sensitivity, specificity, and flexibility, this array kit empowers researchers to deepen their understanding of immune regulation and inflammatory pathways in health and disease.
| Product Sku: | SARB0040 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 80 |
| Gene Symbols: | ANG, BDNF, CCL1, CCL11, CCL13, CCL15, CCL17, CCL18, CCL2, CCL20|CCL22, CCL23, CCL24, CCL26, CCL4, CCL5, CCL7, CCL8, CSF1, CSF2, CSF3, CX3CL1, CXCL1, CXCL10, CXCL12, CXCL13, CXCL2, CXCL3, CXCL5, CXCL6, CXCL8, CXCL9, EGF, FGF4, FGF6, FGF7, FGF9, FLT3LG, GDNF, HGF, IFNG, IGF1, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IL10, IL12A, IL13, IL15, IL16, IL1A, IL1B, IL2, IL3, IL4, IL5, IL6, IL7, KITLG, LEP, LIF, LTA, MIF, NTF3, NTF4, OSM, PDGFB, PGF, PPBP, SPP1, TGFB1, TGFB2, TGFB3, THPO, TIMP1, TIMP2, TNF, TNFRSF11B, TNFSF14, TPO, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Angiogenin
| BDNF
| BLC (CXCL13)
| CK beta 8-1 (CCL23)
| EGF
|
ENA-78 (CXCL5)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| Eotaxin-3 (CCL26)
| FGF-4
|
FGF-6
| FGF-7 (KGF)
| FGF-9
| Flt-3 Ligand
| Fractalkine (CX3CL1)
|
GCP-2 (CXCL6)
| GCSF
| GDNF
| GM-CSF
| GRO alpha (CXCL1)
|
GRO alpha/beta/gamma
| HGF
| I-309 (TCA-3/CCL1)
| IFN-gamma
| IGF-1
|
IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
| IL-1 alpha (IL-1 F1)
|
IL-1 beta (IL-1 F2)
| IL-2
| IL-3
| IL-4
| IL-5
|
IL-6
| IL-7
| IL-8 (CXCL8)
| IL-10
| IL-12 p40/p70
|
IL-13
| IL-15
| IL-16
| IP-10 (CXCL10)
| Leptin
|
LIF
| LIGHT (TNFSF14)
| MCP-1 (CCL2)
| MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
|
MCP-4 (CCL13)
| M-CSF
| MDC (CCL22)
| MIF
| MIG (CXCL9)
|
MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
| MIP-3 alpha (CCL20)
| NAP-2 (PPBP/CXCL7)
| NT-3
|
NT-4
| Oncostatin M
| Osteopontin (SPP1)
| Osteoprotegerin (TNFRSF11B)
| PARC (CCL18)
|
PDGF-BB
| PLGF
| RANTES (CCL5)
| SCF
| SDF-1 alpha (CXCL12 alpha)
|
TARC (CCL17)
| TGF beta 1
| TGF beta 2
| TGF beta 3
| Thrombopoietin (TPO)
|
TIMP-1
| TIMP-2
| TNF alpha
| TNF beta (TNFSF1B)
| VEGF-A
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C5 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
