Description
Human EIF5B Recombinant Protein (N-GST)
Human EIF5B Recombinant Protein (N-GST) is a recombinant protein expressed in E. coli, spanning Thr871-Ile1220, with an N-terminal GST tag. Predicted molecular weight: 66.38 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Eukaryotic translation initiation factor 5B is a ~138 kDa protein. Plays a role in translation initiation. For research use only.
Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after formation of the 80S initiation complex. Its GTPase activity is not essential for ribosomal subunits joining, but GTP hydrolysis is needed for eIF1A (EIF1AX) ejection quickly followed by EIF5B release to form elongation-competent ribosomes.
| Product Name: | Human EIF5B Recombinant Protein (N-GST) |
| SKU: | GXRP10748 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | EC:3.6.5.3, EIF5B, Eukaryotic translation initiation factor 5B, IF2, KIAA0741, Translation initiation factor IF-2, eIF-5B |
| Tag: | N-GST |
| Expression Host: | E. coli |
| Protein Length: | Thr871-Ile1220 |
| Calculated MW: | 66.38 kDa |
| UniProt: | O60841 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
