The Human Endoglin CLIA Kit is specifically designed for the quantitative measurement of endoglin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, providing accurate and reproducible results for a variety of research applications.Endoglin is a key protein involved in angiogenesis and vascular development, playing a critical role in various pathologies such as cancer, cardiovascular diseases, and inflammation.
By detecting endoglin levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.With its advanced technology and user-friendly protocols, the Human Endoglin CLIA Kit from Assay Genie is the perfect tool for studying endoglin biology and its implications in human health and disease. Don't miss this opportunity to enhance your research with reliable and precise measurements of endoglin levels. Order your kit today!
Product Name:
Human ENG (Endoglin) CLIA Kit
SKU:
HUES00060
Target:
Human ENG (Endoglin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ENG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ENG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ENG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ENG. You can calculate the concentration of Human ENG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
100-116
95-111
Average (%)
105
107
102
1:4
Range (%)
92-106
96-109
98-110
Average (%)
99
102
104
1:8
Range (%)
86-99
94-104
103-114
Average (%)
92
99
108
1:16
Range (%)
88-100
83-97
98-114
Average (%)
93
90
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
101
EDTA plasma (n=5)
87-97
92
Cell culture media (n=5)
97-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
203.86
498.93
1719.99
195.36
518.52
1768.97
Standard deviation
17.67
59.82
140.01
16.18
39.82
117.81
C V (%)
8.67
11.99
8.14
8.28
7.68
6.66
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ENG concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ENG in samples. No significant cross-reactivity or interference between Human ENG and analogues was observed.
