The Human GAD1 (Glutamate Decarboxylase 1) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of GAD1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a wide range of research applications.GAD1, also known as glutamic acid decarboxylase 1, is an enzyme responsible for the conversion of glutamate to GABA (gamma-aminobutyric acid) in the brain. GAD1 plays a crucial role in the regulation of neurotransmission and is involved in various neurological conditions, including epilepsy, schizophrenia, and Parkinson's disease.
Therefore, measuring GAD1 levels can provide valuable insights into the pathophysiology of these disorders and facilitate the development of novel therapeutic strategies. Overall, the Human GAD1 CLIA Kit is a valuable tool for researchers studying the role of GAD1 in neurological disorders and seeking to advance our understanding of the mechanisms underlying these conditions.
Product Name:
Human GAD1 (Glutamate Decarboxylase 1) CLIA Kit
SKU:
HUES01242
Target:
Human GAD1 (Glutamate Decarboxylase 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GAD1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAD1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAD1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GAD1. You can calculate the concentration of Human GAD1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
85-101
101-121
Average (%)
104
92
110
1:4
Range (%)
97-113
98-110
99-111
Average (%)
105
104
105
1:8
Range (%)
86-97
95-107
101-115
Average (%)
91
101
108
1:16
Range (%)
89-105
95-110
91-108
Average (%)
96
102
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-113
103
EDTA plasma (n=5)
98-114
105
Cell culture media (n=5)
102-114
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
2.4
9.36
1.01
2.23
10.04
Standard deviation
0.12
0.28
0.9
0.1
0.16
1.13
C V (%)
12.12
11.67
9.62
9.9
7.17
11.25
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GAD1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GAD1 in samples. No significant cross-reactivity or interference between Human GAD1 and analogues was observed.
