The Human Gastrin GT ELISA Kit is specifically designed for the precise measurement of gastrin levels in human samples including serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Gastrin is a key hormone involved in regulating gastric acid secretion and plays a vital role in the digestive process. Abnormal levels of gastrin have been associated with various gastrointestinal disorders such as peptic ulcers, gastrinomas, and Zollinger-Ellison syndrome.
Therefore, the Human Gastrin GT ELISA Kit is essential for studying these conditions and developing potential treatment strategies.With its reliable performance and user-friendly protocols, the Human Gastrin GT ELISA Kit is a valuable tool for researchers looking to explore the role of gastrin in health and disease.
Product Name:
Human Gastrin (GT) ELISA Kit
Product Code:
HUDL01282
Size:
96 Assays
Target:
GT
Synonyms:
GAST, GAS, Gastrin component I, Big gastrin, Gastrin component II, Gastrin component III
Detection Method:
Competition
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
Subramaniam et al.
Levothyroxine Absorption Test – Results and Follow Up of Patients from a Single Center
Indian Journal of Endocrinology and Metabolism 2023
