The Rat GT (Gastrin) ELISA Kit is a specialized assay designed for the precise quantification of Gastrin levels in various biological samples obtained from rats. Gastrin is an essential peptide hormone that plays a crucial role in stimulating gastric acid secretion and regulating gastrointestinal functions, including gastric motility and mucosal growth. Its functions extend to the modulation of digestive processes and interaction with other hormones in the gastrointestinal tract. This ELISA kit provides researchers with a reliable and accurate method to measure Gastrin concentrations, enabling a better understanding of its role in regulating gastric acid production, gut motility, and mucosal integrity. By accurately determining Gastrin levels, researchers can explore the hormone's involvement in various physiological processes, including digestion, and its implications in gastrointestinal disorders and diseases. Manufactured under strict quality control standards, our Rat GT ELISA Kit ensures robust performance and delivers reproducible results. The exceptional sensitivity and specificity of this kit make it a valuable tool for research studies focusing on Gastrin's functions and its interactions within the gastrointestinal system. Its user-friendly design further simplifies the assay procedure, making it an excellent choice for researchers investigating the physiological roles of Gastrin in gastrointestinal health and disease.
Product Name:
Rat GT (Gastrin) ELISA Kit
SKU:
AEES00422
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
